Cell Culture

不同细胞系的培养条件

Caco2

DMEM, 20%FBS, 1% Pen-Strep

THP-1

悬浮细胞

1640, 10%FBS, 1% Pen-Strep

HEK293T

DMEM, 10%FBS, 1% Pen-Strep
用培养基吹下

RAW264.7

DMEM, 10%FBS, 1% Pen-Strep
用培养基吹下

Hela

DMEM, 10%FBS, 1% Pen-Strep
消化4min

A549

HCT116

Huh7

DMEM, 10%FBS, 1% Pen-Strep, 额外补充谷氨酰胺, MEM-NEAA, HEPES
消化4min

3T3L1

mPASMC

F12, 10%FBS, 1% Pen-Strep
消化2min

抗支原体污染

InvivoGen, Primocin®, 50 mg/mL, 500x