Accucore C30
Accucore™ C30 HPLC Columns, 2.6 μm, 2.1 μm X 150 mm
volumn = π x (2.1mm/2)2 x 150mm = 520 mm3 = 0.52 ml
0.35 ml/min 乙腈冲洗20个柱体积, 0.52 x 20 / 0.35 = 30 min
0.35 ml/min 乙腈水一比一冲洗10个柱体积, 0.52 x 10 / 0.35 = 15 min
0.35 ml/min lipidomics流动相平衡30min
最后95%A, 5%B, 0.35ml/min, 柱压为360